BBa_K1975008

BBa_K1975008 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1975008

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Joachim Steen Larsen

Date created: 2016-10-12 11:00:00

Date modified: 2016-10-13 05:12:52

Beta-Lactamase

Details

• Types: DnaRegion
• Roles: Reporter; engineered_region
• Sequences: BBa_K1975008_sequence (Version 1)

Description

This part contain a beta-lactamase. The function of this enzyme is to degrade beta-lactam and other beta-lactam antibiotics. The way the enzyme does this is by enzymatic cleavage of the beta-lactam ring. This cleavage will open the ring and the molecule will loose its antibacterial effect.
Because of this activity, this enzyme is a very good reporter gene for Escherichia coli. When the E. coli cells contain this BioBrick the cells will survive to grow in media that contain beta-lactam. Cells without the BioBrick will die because beta-lactam will inhibit the cell wall biosynthesis making division of the cells impossible.

Notes

The sequence was obtained by PCR from the shuttle vector pHT254.

Source

This sequence is obtained from the vector pHT254 by PCR. pHT254 is a shuttle vector for the use in E. coli and Bacillus subtilis.

Other Properties

• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1975008/1

Member of these Collections

• iGEM Parts Registry