BBa_K1979005

BBa_K1979005 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1979005
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: MA Xinyi
Date created: 2016-09-16 11:00:00
Date modified: 2016-09-17 12:37:49

CBD-PBP5



Types
DnaRegion

Roles
Generator

engineered_region

Sequences BBa_K1979005_sequence (Version 1)

Description

This part codes for CBD (cellulose binding domain)- PBP5 protein.

Notes

Histidine tag is added for affinity purification to harvest the proteins. g10-L is a strong RBS in E. coli but also well suited for foreign gene expression.The effect of the ribosome binding site (RBS) on in vitro translation has been investigated extensively (Karig et al. 2012; Lentini et al. 2013; Takahashi et al. 2013). Most important for an efficient reaction is a defined distance (4-9 bases) of the RBS to the following start codon (Karig et al. 2012; Lentini et al. 2013), but the strength of the RBS has a minor effect as long as it is not too weak (Karig et al. 2012; Takahashi et al. 2013).

Source

The PBP5 is from the E.coli's genes, CBD sequence is retrieved from GenBank, and the promoter is from part BBa_J23101.

Sequence Annotation Location Component / Role(s)
J23101
g10-L rbs
his
T7
CBD
PBP5
T7 terminator
1,35
58,64
84,101
132,164
174,320
321,1529
1620,1667
feature/promoter promoter
feature/rbs ribosome_entry_site
feature/tag tag
feature/tag tag
feature/protein CDS
feature/protein CDS
stop_codon feature/stop
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1979005/1