Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Sam Garforth
Date created: 2016-10-10 11:00:00
Date modified: 2016-10-12 02:23:43
TAT Copper Storage Protein 1
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K1980000_sequence (Version 1) |
Description
Copper Storage protein 1 (Csp1) is a tetrameric copper storage protein found in the periplasm of Methylosinus trichosporium OB3b. We investigated whether this part could act as a copper chelator when expressed in E. coli.We modified the protein by adding a TAT signal peptide from the E. coli enzyme CueO in place of the native TAT sequence and a C terminal his tag in an attempt to direct the protein to the periplasm.
Notes
Codon optimised for E. coli. TAT sequence changed to an E. coli TAT sequence.Source
The source organism for the main protein sequence is Methylosinus trichosporium OB3b. The TAT sequence originate from E. coli multi copper oxidase enzyme CueO. We ordered it as codon optimised DNA from IDT.| Sequence Annotation | Location | Component / Role(s) |
| TAT signal peptide Csp1 Hexa Histidine stop | 1,84 85,450 450,468 469,474 | sequence_feature feature/misc CDS feature/cds feature/tag tag feature/stop stop_codon |