Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Sam Garforth
Date created: 2016-10-10 11:00:00
Date modified: 2016-10-12 02:35:29
TAT Copper Storage Protein 1 sfGFP
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K1980001_sequence (Version 1) |
Description
Copper Storage protein 1 (Csp1) is a tetrameric copper storage protein found in the periplasm of Methylosinus trichosporium OB3b. We investigated whether this part could act as a copper chelator when expressed in E. coli. We modified the protein by adding a TAT signal peptide from the E. coli enzyme CueO in place of the native TAT sequence and added a c terminal sfGFP fluorescence protein with a C terminal his tag in an attempt to direct the protein to the periplasm. The Csp1 and sfGFP are separated by a short hydrophilic, flexible linker.Notes
Codon optimised for E. coli. linker added in order to allow the Csp1 and sfGFP to ideally fold separately. Hexahistidine purification tag on c-terminus to maintain TAT sequence.Source
The source organism for the main protein sequence is Methylosinus trichosporium OB3b. The TAT sequence originate from E. coli multi copper oxidase enzyme CueO. We ordered it as codon optimised DNA from IDT.| Sequence Annotation | Location | Component / Role(s) |
| TAT signal peptide Csp1 linker sfGFP Hexahistidine Double stop | 1,84 85,450 451,468 469,1179 1180,1197 1198,1203 | sequence_feature feature/misc feature/cds CDS sequence_feature feature/misc feature/cds CDS feature/tag tag stop_codon feature/stop |