Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Sam Garforth
Date created: 2016-10-10 11:00:00
Date modified: 2016-10-20 07:26:41
MymT sfGFP
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K1980003_sequence (Version 1) |
Description
MymT is a small prokaryotic copper metallothein discovered in Mycobacterium tuberculosis. It is believed that the protein may help the bacterium survive copper toxicity, though deleting the gene had no effect on pathogenicity in mice. It can bind up to 7 copper ions but has a preference for 4-6. This version has a C terminal sfGFP with a C terminal hexahistidine tag for purification. The MymT and sfGFP are separated by a small flexible linker. This part has been codon optimised for E. coli.Notes
This protein has a C terminal hexahistidine tag for purification and has been codon optimised for E. coli.Source
Mycobacterium tuberculosis. Ordered as codon optimised DNA.| Sequence Annotation | Location | Component / Role(s) |
| MymT linker sfGFP Hexahistidine Double stop | 1,159 160,177 178,888 889,906 906,912 | feature/cds CDS sequence_feature feature/misc CDS feature/cds feature/tag tag feature/stop stop_codon |