Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Yulei Dang, Xinhao Song
Date created: 2016-10-13 11:00:00
Date modified: 2016-10-14 06:23:15
lsrA
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K1981001_sequence (Version 1) |
Description
Autoinducer 2 import ATP-binding protein LsrA. Part of the ABC transporter complex LsrABCD involved in autoinducer 2 (AI-2) import. Regulation of uptake and processing of the quorum-sensing AI-2. Responsible for energy coupling to the transport system (Probable). This protein is essential for aerobic growth. In the absence of AI-2, repressed by LsrR. Induced by AI-2, via release of the LsrR repressor. In the absence of glucose, induced by cAMP-CRP by direct binding to the upstream region of the lsr promoter.Notes
Point mutation was performed to mutate the restriction site of XbaI at 192 bp. Condon optimization was performed to meet the manufacture requirement of IDT to synthesize the DNA fragment.Source
Sequence was acquired from genomic of Bacillus thuringiensis serovar thuringiensis str. IS5056. Part was chemically synthesized.| Sequence Annotation | Location | Component / Role(s) |
| ATG Start Codon lsrA Coding Region TGA Stop Codon | 1,3 1,1200 1198,1200 | start_codon feature/start feature/cds CDS feature/stop stop_codon |