Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Zexu Li
Date created: 2016-09-11 11:00:00
Date modified: 2016-10-18 10:38:55
CRY2-VP64(Prokaryotic LACE system)
| Types | DnaRegion |
| Roles | engineered_region Device |
| Sequences | BBa_K1982005_sequence (Version 1) |
Description
The CRY2/CIBN interaction is entirely genetically encoded. The binding reverses within minutes in the dark, allowing rapid shutoff of transcription by placing samples in the dark. This fusion protein is for use in LACE(light-activated CRISPR/Cas9 effector) system, and a VP64 fused to its C terminus. To regulate DNA transcription by blue light, the system is based on CRY2/CIBN interaction in which a light-mediated protein interaction brings together two protein (tCas9 and an activation domain VP64) . If we remove the stimulation of blue light, dark reversion of CRY2 will dissociate the interaction with CIBN and shut off transcription.Notes
noSource
CRY2 and CIB1 are extracted from Arabidopsis thaliana. tCas9 is from GE Share company. VP64 is synthetic construct.| Sequence Annotation | Location | Component / Role(s) |
| Prokaryotic rbs ATG CRY2 SV40 NLS VP16 VP16 VP16 VP16 Linker HA FLAG TAATAA | 1,18 19,21 22,1854 1855,1875 1876,1914 1915,1953 1954,1992 1993,2031 2032,2037 2037,2063 2071,2094 2095,2100 | ribosome_entry_site feature/rbs start_codon feature/start CDS feature/protein feature/misc sequence_feature feature/misc sequence_feature feature/misc sequence_feature sequence_feature feature/misc sequence_feature feature/misc sequence_feature feature/misc feature/tag tag tag feature/tag stop_codon feature/stop |