Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Bowman Clark
Date created: 2016-10-13 11:00:00
Date modified: 2016-10-14 09:44:11
his-tagged ppx2 homologue w/ Anderson Promoter J23106
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K1988001_sequence (Version 1) |
Description
Name: C- Terminus His-tagged Exopolyphosphatase with Anderson promoter J23106Locus tag: MLP_44770
Function:
There are two types of exopolyphosphatase, PPX1 and PPX2, both of which mediate the hydrolysis of the terminal phosphate of a polyphosphate chain. [1]
In Microlunatus phosphovorus: While most Actinobacteria species have both PPX1 and PPX2, M. phosphovorus only has one PPX2 homolog. [1]
Cofactors and Modifications: There are currently no known cofactors and modifications of the PPX2 homolog in M. phosphovorus.
3-D Structure:
C-score: 1.01 The three-dimensional structure was predicted using the I-TASSER software [3]. All images shown are the models with the highest confidence, which is quantitatively measured with a C-score that typically falls within the range [-5,2], in which 2 represents the highest level of confidence, and -5 represents the lowest level of confidence.
Gene length: 945 base pairs [4]
Protein size:33.0 kDa [4]
pH Range The PPX2 homolog in M. phosphovorus has yet to be characterized, and so there is no data for the pH range in which it functions.
Temperature Range The PPX2 homolog in M. phosphovorus has yet to be characterized, and so there is no data for the temperature range in which it functions.
References
[1] A. Kawakoshi, H. Nakazawa, J. Fukada, M. Sasagawa, Y. Katano, S. Nakamura, A. Hosoyama, H. Sasaki, N. Ichikawa, S. Hanada, Y. Kamagata, K. Nakamura, S. Yamazaki and N. Fujita, "Deciphering the Genome of Polyphosphate Accumulating Actinobacterium Microlunatus phosphovorus", DNA Research, vol. 19, no. 5, pp. 383-394, 2012.
[2]
[3] Yang, R. Yan, A. Roy, D. Xu, J. Poisson and Y. Zhang, "The I-TASSER Suite: protein structure and function prediction", Nature Methods, vol. 12, no. 1, pp. 7-8, 2014.
[4] "ppx - Exopolyphosphatase - Microlunatus phosphovorus (strain ATCC 700054 / DSM 10555 / JCM 9379 / NBRC 101784 / NCIMB 13414 / VKM Ac-1990 / NM-1) - ppgK gene & protein", Uniprot, 2016. [Online]. Available: http://www.uniprot.org/uniprot/F5XI06. [Accessed: 22- Jul- 2016].
Notes
Genomic sequence optimized for E. Coli K-12 using IDT Codon optimizer.Source
Genomic sequence from paper 'Deciphering the Genome of Polyphosphate Accumulating Actino bacterium Microlunatus Phosphovorus' by Kawakoshi, et. al.| Access | Instance | Definition |
| public public public public | BBa_J23106 BBa_J34803 BBa_K1988000 BBa_K844000 | BBa_J23106 BBa_J34803 BBa_K1988000 BBa_K844000 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J23106 BBa_J34803 BBa_K1988000 BBa_K844000 | 1,35 44,56 65,1006 1015,1050 | BBa_J23106 BBa_J34803 BBa_K1988000 BBa_K844000 |