BBa_K1989005

BBa_K1989005 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1989005
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Yiming Dong
Date created: 2016-10-12 11:00:00
Date modified: 2016-10-14 02:43:59

Quadruple spytag with SUP and His-tag



Types
DnaRegion

Roles
CDS

Coding

Sequences BBa_K1989005_sequence (Version 1)

Description

In the last few years, hydrogens made from natural or synthetic polymers have been investigated due to their extensive application in clinical medicine and synthetic biology. Compared to traditional biological material, protein-based multifunctional biological material is low-cost, facile and eco-friendly. However, strategies for assembling 3D molecular networks synthesized only by protein molecular remain underdeveloped. The reason why investigating this technology is still tough is lack of protein-based cross linking agents. Inspired by the self-catalysis of isopeptide bond between Lys and Asp in Streptococcus pyogenes fibronectin-binding protein FbaB, researchers split the catalytic domain and obtained two peptide called SpyTag(the short one) and SpyCatcher(the long one) which are able to form isopeptide bond with the other without any assistant. By fusing SpyTag and SpyCatcher with functional domains respectively, researchers solved the problem tactfully. In order to using SpyTag and SpyCatcher system as scaffold, we fused three SpyTag spaced by (VPGVG)4 with 6xHistag in N-terminal and another functional protein called Super Uranyl-binding Protein(SUP) in C-terminal.

Notes

The length of the linker between spytag and SUP may affect the physical and chemical property of hydrogel.

Source

Inspiring from the self-catalysis of isopeptide bond between Lys and Asp in Streptococcus pyogenes fibronectin-binding protein FbaB, researchers split the catalytic domain and obtain two peptide called Spytag(the short one) and Spycatcher(the long one) which are able to form isopeptide bond with the other without any assistant. SUP is a completely artificial protein from structure calculating to function modifying.

Sequence Annotation Location Component / Role(s)
his-tag
Quadruple spytag
SUP
30,68
69,443
528,764
tag feature/tag
feature/protein CDS
feature/protein CDS
igem#experience
None
 
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1989005/1