Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Yiming Dong
Date created: 2016-10-08 11:00:00
Date modified: 2016-10-09 03:34:52
Triple spytag with SUP, FlAsH-tag and signal peptide NprE
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K1989013_sequence (Version 1) |
Description
The fused protein NprE-Histag-3A-SUP-FlAsHtag possess both isopeptide bond forming function, uranyl-binding ability and secretion . Thus, using NprE-Histag-3A-SUP-FlAsHtag as a part of hydrogel formation, we can obtain our multifunctional biomaterial.Notes
The length of the linker between spytag and SUP may affect the physical and chemical property of hydrogel. As several attributes of our target proteins, especially their folding state in cells, we were unable to rationally design or choose a signal peptide for each. Nevertheless, we could build a Signal Peptide Repertory to screen for the best SP candidate. Our ultimate goal was to select the most appropriate ones from a huge amount of signal peptides.Source
Inspiring from the self-catalysis of isopeptide bond between Lys and Asp in Streptococcus pyogenes fibronectin-binding protein FbaB, researchers split the catalytic domain and obtain two peptide called Spytag(the short one) and Spycatcher(the long one) which are able to form isopeptide bond with the other without any assistant. SUP is a completely artificial protein from structure calculating to function modifying.| Sequence Annotation | Location | Component / Role(s) |
| RBS Signal peptide NprE Spytag-Spytag-Spytag-SUP FlAsH-tag | 30,59 60,140 144,751 756,794 | feature/rbs ribosome_entry_site feature/protein CDS feature/protein CDS tag feature/tag |