BBa_K1989040

BBa_K1989040 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1989040
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Yiming Dong
Date created: 2016-10-10 11:00:00
Date modified: 2016-10-11 10:55:04

Triplespytag with mRFP, FlAsH-tag and signal peptide ImdA



Types
DnaRegion

Roles
Coding

CDS

Sequences BBa_K1989040_sequence (Version 1)

Description

The fused protein ImdA-3A-mRFP-FlAsHtag possess both isopeptide bond forming function, uranyl-binding ability and secretion. Thus, using ImdA-3A-mRFP-FlAsHtag as a part of hydrogel formation, we can obtain our multifunctional biomaterial.

Notes

The length of the linker between spytag may affect the physical and chemical property of hydrogel. As several attributes of our target proteins, especially their folding state in cells, we were unable to rationally design or choose a signal peptide for each. Nevertheless, we could build a Signal Peptide Repertory to screen for the best SP candidate. Our ultimate goal was to select the most appropriate ones from a huge amount of signal peptides.

Source

Inspiring from the self-catalysis of isopeptide bond between Lys and Asp in Streptococcus pyogenes fibronectin-binding protein FbaB, researchers split the catalytic domain and obtain two peptide called Spytag(the short one) and Spycatcher(the long one) which are able to form isopeptide bond with the other without any assistant.

Sequence Annotation Location Component / Role(s)
RBS
Signal peptide ImdA
Spytag-Spytag-Spytag-mRFP
FlAsH-tag
stop
30,61
62,151
155,1168
1172,1210
1208,1210
ribosome_entry_site feature/rbs
feature/protein CDS
CDS feature/protein
tag feature/tag
stop_codon feature/stop
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1989040/1