BBa_K199032

BBa_K199032 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K199032
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Olivia Ho-Shing
Date created: 2009-07-12 11:00:00
Date modified: 2015-05-08 01:11:18

pBad-CCAUC Suppressor tRNA (10-bp Anticodon)



Types
DnaRegion

Roles
mature_transcript_region

RNA

Sequences BBa_K199032_sequence (Version 1)

Description

Causes low expression of a suppressor tRNA that suppresses a 5-bp codon CCAUC, Part K199008.

Notes

We used the pBad promoter to transcribe lower levels of tRNA in a high copy plasmid, pSB1A2.

Source

The pBad promoter is from the BioBrick Parts registry. Suppressor tRNA was made by de novo synthesis from single-stranded oligos. Based on the paper by Anderson et al. 2002.

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BBa_K199032/1