Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Alyndria Thompson
Date created: 2009-07-12 11:00:00
Date modified: 2015-05-08 01:11:19
Tet Resistance with CGGUC Addition
| Types | DnaRegion |
| Roles | engineered_region Reporter |
| Sequences | BBa_K199035_sequence (Version 1) |
Description
This Tet reporter gene is a modification of BBa_J31007; it has a 5-bp addition, CGGUC, after the start codon and before the rest of the gene. This is used in conjunction with the CGGUC tRNA (Part K199028). These CGGUC tRNAs suppress the frameshift caused by this 5-bp addition in the reporter.Notes
We positioned the start codon, ATG, before the 5-bp codon and continued with the rest of the Tet gene. This way, the RBS wouldn't translate the reporter gene without reading over the 5-base codon first. In order to ligate our edited 5' sequence to the unchanged downstream portion of the Tet gene, we utilized the restriction enzyme BamHI that cuts at a single site 290-bp into BBa_J31007.Source
The template of the gene comes from Part BBa_J31007. We designed a forward primer that included ATG, the 5-bp addition CGGTC and the first 20 nucleotides on the 5' end of Tet after ATG. This made the edited 5' sequence for our Tet.| Sequence Annotation | Location | Component / Role(s) |
| 5-bp CGGUC Addition BamH1 restriciton site | 4,9 295,300 | feature/misc sequence_feature sequence_feature feature/misc |