BBa_K199058

BBa_K199058 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K199058
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Olivia Ho-Shing
Date created: 2009-08-04 11:00:00
Date modified: 2015-05-08 01:11:19

CCACU tRNA with RFP Reporter



Types
DnaRegion

Roles
engineered_region

Device

Sequences BBa_K199058_sequence (Version 1)

Description

Suppressor tRNA expressed by the medium-weak promoter, pBad, upstream of RFP with a 5-bp insertion, CCACU. The tRNA is designed to suppress the insertion, allowing the appropriate translation of functional RFP.

Notes

See the following parts for more information: CCAUC tRNA, pBad-tRNA and RFP with 5-bp addition. We wanted to create this composite so that the tRNA and reporter would be replicated in the same plasmid, and to be more efficient than a double transformation.

Source

From the BioBrick Parts Registry. The tRNA was constructed by oligo assembly, and mutated the RFP reporter, E1010, to add the 5-bp insertion by PCR.

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BBa_K199058/1