Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Olivia Ho-Shing
Date created: 2009-08-04 11:00:00
Date modified: 2015-05-08 01:11:19
CCACU tRNA with RFP Reporter
| Types | DnaRegion |
| Roles | engineered_region Device |
| Sequences | BBa_K199058_sequence (Version 1) |
Description
Suppressor tRNA expressed by the medium-weak promoter, pBad, upstream of RFP with a 5-bp insertion, CCACU. The tRNA is designed to suppress the insertion, allowing the appropriate translation of functional RFP.Notes
See the following parts for more information: CCAUC tRNA, pBad-tRNA and RFP with 5-bp addition. We wanted to create this composite so that the tRNA and reporter would be replicated in the same plasmid, and to be more efficient than a double transformation.Source
From the BioBrick Parts Registry. The tRNA was constructed by oligo assembly, and mutated the RFP reporter, E1010, to add the 5-bp insertion by PCR.| Access | Instance | Definition |
| public public public public public | BBa_I13453 BBa_K199002 BBa_R0010 BBa_B0034 BBa_K199005 | BBa_I13453 BBa_K199002 LacI BBa_B0034 BBa_K199005 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_I13453 BBa_K199002 BBa_R0010 BBa_B0034 BBa_K199005 | 1,130 139,281 290,489 498,509 516,1226 | BBa_I13453 BBa_K199002 LacI BBa_B0034 BBa_K199005 |