BBa_K199076

BBa_K199076 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K199076
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Clifton Davis
Date created: 2009-09-19 11:00:00
Date modified: 2015-05-08 01:11:19

RBS RFP with CUACU Addition



Types
DnaRegion

Roles
Reporter

engineered_region

Sequences BBa_K199076_sequence (Version 1)

Description

This RBS RFP reporter gene is a modification of BBa_E1010 with BBa_B0034; it has a 5-bp addition, CUACU, after the start codon and before the rest of the gene. This is used in conjunction with the CUACU tRNA(Part K199046). These CUACU tRNAs suppress the frameshift caused by this 5-bp addition in the reporter.

There is also a 25 bp addition after the double stop of the gene and before the Bio Brick Suffix. This addition is presumed to not effect the translation of the protein.

Sequence and Features

Notes

None

Source

The template of the gene comes from Part BBa_E1010. We designed a forward primer that included RBS,ATG, the 5-bp addition CTACT and the first 20 nucleotides on the 5' end of RFP after ATG. This made the edited 5' sequence for our RFP.

Sequence Annotation Location Component / Role(s)
NcoI Restriction Site
5-bp CUACU Addition
25-bp addition
442,442
705,729
705,729
sequence_feature feature/misc
sequence_feature feature/misc
feature/misc sequence_feature
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/myers
 
synbiohub#ownedBy
user/james
 
synbiohub#topLevel
BBa_K199076/1