Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Clifton Davis
Date created: 2009-09-19 11:00:00
Date modified: 2015-05-08 01:11:19
RBS RFP with CUACU Addition
| Types | DnaRegion |
| Roles | Reporter engineered_region |
| Sequences | BBa_K199076_sequence (Version 1) |
Description
This RBS RFP reporter gene is a modification of BBa_E1010 with BBa_B0034; it has a 5-bp addition, CUACU, after the start codon and before the rest of the gene. This is used in conjunction with the CUACU tRNA(Part K199046). These CUACU tRNAs suppress the frameshift caused by this 5-bp addition in the reporter.There is also a 25 bp addition after the double stop of the gene and before the Bio Brick Suffix. This addition is presumed to not effect the translation of the protein.
Sequence and Features
Notes
NoneSource
The template of the gene comes from Part BBa_E1010. We designed a forward primer that included RBS,ATG, the 5-bp addition CTACT and the first 20 nucleotides on the 5' end of RFP after ATG. This made the edited 5' sequence for our RFP.| Sequence Annotation | Location | Component / Role(s) |
| NcoI Restriction Site 5-bp CUACU Addition 25-bp addition | 442,442 705,729 705,729 | sequence_feature feature/misc sequence_feature feature/misc feature/misc sequence_feature |