BBa_K1991005

BBa_K1991005 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1991005

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Chen, Pei-En

Date created: 2016-10-11 11:00:00

Date modified: 2016-10-12 02:57:28

LO-AOX1-His (Lpp-OmpA-Alcohol Oxidase)

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K1991005_sequence (Version 1)

Description

A study showed that Lpp-OmpA (LO) hybrid can direct the heterologous protein GFP to the external surface of E. coli (Enzyme Microb Technol. 2001). Bacillus lipase (J Microbiol. 2014) and Fungi xylanase (Curr Microbiol. 2015) were demonstrated to be displayed on the cell surface of E. coli and maintained the functional enzyme activities. We???d like to display the AOX enzyme on bacterial surface by fusing with Lpp-OmpA and apply to the electrochemical analyzer by depositing on the test strip.

Notes

No

Source

E. coli (Lpp-OmpA) & Pichia pastoris (AOX)

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
Lpp-OmpA	1,441	feature/cds, CDS
AOX1	442,2430	CDS, feature/cds
6X His Tag	2452,2469	tag, feature/tag
T7 terminator	2537,2583	sequence_feature, feature/misc

Other Properties

• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1991005/1

Member of these Collections

• iGEM Parts Registry