Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Chen, Pei-En
Date created: 2016-10-11 11:00:00
Date modified: 2016-10-12 02:57:28
LO-AOX1-His (Lpp-OmpA-Alcohol Oxidase)
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K1991005_sequence (Version 1) |
Description
A study showed that Lpp-OmpA (LO) hybrid can direct the heterologous protein GFP to the external surface of E. coli (Enzyme Microb Technol. 2001). Bacillus lipase (J Microbiol. 2014) and Fungi xylanase (Curr Microbiol. 2015) were demonstrated to be displayed on the cell surface of E. coli and maintained the functional enzyme activities. We???d like to display the AOX enzyme on bacterial surface by fusing with Lpp-OmpA and apply to the electrochemical analyzer by depositing on the test strip.Notes
NoSource
E. coli (Lpp-OmpA) & Pichia pastoris (AOX)| Sequence Annotation | Location | Component / Role(s) |
| Lpp-OmpA AOX1 6X His Tag T7 terminator | 1,441 442,2430 2452,2469 2537,2583 | feature/cds CDS CDS feature/cds tag feature/tag sequence_feature feature/misc |