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BBa_K199110

BBa_K199110 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K199110

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Mary Gearing

Date created: 2009-12-04 12:00:00

Date modified: 2015-05-08 01:11:20

pLac-RBS-CCCUC-RFP-pLpp-CCCUC tRNA

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Details

Types	DnaRegion
Roles	engineered_region Device
Sequences	BBa_K199110_sequence (Version 1)

Description

Reporter construct ligated upstream of a suppressor tRNA. The tRNA suppresses the 5 bp insert in the RFP gene. The promoter controlling tRNA production is pLpp, one of the strongest promoters in E. coli.

Notes

n/a

Source

pLpp- genomic sequence of E. coli

Components

Access	Instance	Definition
public public public public public	BBa_R0010 BBa_B0034 BBa_K199011 BBa_K199106 BBa_K199000	LacI BBa_B0034 BBa_K199011 BBa_K199106 BBa_K199000

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_R0010 BBa_B0034 BBa_K199011 BBa_K199106 BBa_K199000	1,200 209,220 227,937 946,994 1003,1145	LacI BBa_B0034 BBa_K199011 BBa_K199106 BBa_K199000