BBa_K199123

BBa_K199123 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K199123
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Romina Clemente
Date created: 2010-03-08 12:00:00
Date modified: 2015-05-08 01:11:20

autoinducer synthetase for AHL (no LVA) + Unique Site



    • Details

    Types
    DnaRegion

    Roles
    Coding

    CDS

    Sequences BBa_K199123_sequence (Version 1)

    Description

    Synthesizes 3OC6HSL, which binds to LuxR.

    Directly downstream of the ATG start codon is an AsiSI restriction site, a 5 bp-spacer, and an AscI restriction site. These two restriction sites allow for unique coding sequences to easily be swapped out of the gene. These unique coding sequences contain frameshift mutations that can be suppressed by 5-bp tRNA suppressors. Whether or not these frameshift mutations are suppressed determines whether or not LuxI will be transcribed properly.

    Notes

    -

    Source

    V. fischeri [Genbank AF170104]

    Sequence Annotation Location Component / Role(s)
    Unique Site
    		4,24
    		sequence_feature feature/misc

    igem#sampleStatus
    Not in stock
    igem#status
    Unavailable
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K199123/1
     

    Attachments

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