BBa_K199125

BBa_K199125 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K199125

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Olivia Ho-Shing

Date created: 2010-03-16 12:00:00

Date modified: 2015-05-08 01:11:20

LuxI autoinducer synthetase optimized for E. coli

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K199125_sequence (Version 1)

Description

Synthesizes 3OC6HSL, which binds to LuxR. No LVA tag.

Directly downstream of the ATG is 21 base pairs translated into 7 amino acids not seen at the beginning of wild-type LuxI (C0161). These base pairs will be mutated from this "optimized wild-type" sequence to become an AsiSI restriction site, a 5 bp-spacer, and an AscI restriction site. These two restriction sites will allow for unique coding sequences to easily be swapped out of the gene. This "optimized wild-type" sequence acts as a positive control before adding in the restriction sites or additional coding sequences.

Notes

The amino acid sequence is the same as wild-type LuxI, but the DNA sequence optimized for Escherichia coli codon bias. 21 base pairs have been added after the start codon (ATG) that will be altered to create two 8-nt restriction enzyme sites and a spacer in order to add in base pairs directly after the start codon.

Source

Wild-type LuxI gene optimized for E. coli codon bias using GENEART for gene synthesis.

Other Properties

• igem#experience: Works
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K199125/1

Member of these Collections

• iGEM Parts Registry