Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Liam Carroll
Date created: 2016-09-21 11:00:00
Date modified: 2016-10-19 09:23:58
sgRNA with dCas9 binding site sequence 9 and PP7 handle insert
| Types | DnaRegion |
| Roles | RNA mature_transcript_region |
| Sequences | BBa_K1994013_sequence (Version 1) |
Description
For use in CRISPR/Cas9 systems. Contains golden gate adapter at 5' end for inserting target sequence. Also contains second golden gate adapter between truncated terminator and aspA terminator. Both golden gate adapters contain Bsa1 cut sites.Notes
No special considerationsSource
Completely synthetic| Sequence Annotation | Location | Component / Role(s) |
| Sequence 9 dCas9 handle truncated S. pyrogenes terminator 10bp Linker aspA terminator PP7 BP handle 10bp Linker | 1,20 21,62 63,96 97,107 150,189 108,138 139,149 | feature/misc sequence_feature feature/stem_loop stem_loop stem_loop feature/stem_loop feature/misc sequence_feature feature/stem_loop stem_loop stem_loop feature/stem_loop sequence_feature feature/misc |