BBa_K1994016

BBa_K1994016 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1994016
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Liam Carroll
Date created: 2016-09-25 11:00:00
Date modified: 2016-10-19 01:26:31

sgRNA with 5' golden gate adapter and MS2 coat protein binding site



    • Details

    Types
    DnaRegion

    Roles
    RNA

    mature_transcript_region

    Sequences BBa_K1994016_sequence (Version 1)

    Description

    For use in CRISPR/Cas9 systems. Contains golden gate adapter at 5' end for inserting target sequence. Contains a MS2 coat protein binding site in the middle.

    Notes

    No special considerations

    Source

    Synthetic

    Sequence Annotation Location Component / Role(s)
    20nt golden gate adapter
    dCas9 handle
    truncated S. pyrogenes terminator
    5bp linker
    MS2 handle
    5bp linker
    aspA terminator
    		1,20
    21,62
    63,96
    97,101
    102,126
    127,131
    132,172
    		sequence_feature feature/misc
    feature/stem_loop stem_loop
    feature/stem_loop stem_loop
    feature/misc sequence_feature
    feature/stem_loop stem_loop
    feature/misc sequence_feature
    feature/misc sequence_feature

    igem#status
    Planning
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K1994016/1
     

    Attachments

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