Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Liam Carroll
Date created: 2016-09-25 11:00:00
Date modified: 2016-10-19 01:34:26
sgRNA with 5' golden gate adapter and PP7 protein binding site
| Types | DnaRegion |
| Roles | RNA mature_transcript_region |
| Sequences | BBa_K1994017_sequence (Version 1) |
Description
For use in CRISPR/Cas9 systems. Contains golden gate adapter at 5' end for inserting target sequence. Contains a PP7 coat protein binding site in the middle.Notes
No special considerationsSource
Synthetic| Sequence Annotation | Location | Component / Role(s) |
| 20nt golden gate adapter dCas9 handle truncated S. pyrogenes terminator 10bp linker PP7 handle 10bp linker aspA terminator | 1,20 21,62 63,96 97,107 108,139 140,150 150,178 | sequence_feature feature/misc feature/stem_loop stem_loop feature/stem_loop stem_loop feature/misc sequence_feature feature/stem_loop stem_loop sequence_feature feature/misc stem_loop feature/stem_loop |