Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Chushu Zhu
Date created: 2016-10-12 11:00:00
Date modified: 2016-10-13 06:18:45
P+R->sHRP-N->Zif268-> sHRP-C->TER
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K1997012_sequence (Version 1) |
Description
This part is an integrated tool for protein-protein interaction research using split-HRP system as reporter. the "Zif268" subpart can be easily replaced using Golden Gate technique with BsaINotes
We added BsaI sites at the flank of "Zif268" sequence so that this sequence can be easily replacedSource
This part was synthesized by IDT| Sequence Annotation | Location | Component / Role(s) |
| BBa_K1789012 barcode sHRP-N 10aa linker Zif268 10aa linker sHRP-C barcode BBa_B0015 | 1,223 224,228 229,870 871,900 913,1173 1186,1215 1216,1503 1504,1509 1510,1638 | engineered_region feature/BioBrick engineered_tag feature/barcode feature/cds CDS CDS feature/protein feature/cds CDS feature/protein CDS feature/cds CDS engineered_tag feature/barcode engineered_region feature/BioBrick |