BBa_K1997014

BBa_K1997014 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1997014

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Xinyuan Qiu

Date created: 2016-10-12 11:00:00

Date modified: 2016-10-19 06:47:52

GFP10->Zif268->GFP11->RBS->GFP1-9

Details

• Types: DnaRegion
• Roles: Reporter; engineered_region
• Sequences: BBa_K1997014_sequence (Version 1)

Description

This part is an integrated tool for protein-protein interaction research using split-HRP system as reporter. the "FRB-RBS-FKBP" subpart can be easily replaced using Golden Gate technique with BsaI

Notes

We added BsaI sites at the flank of "FRB-RBS-FKBP" sequence so that this sequence can be easily replaced

Source

This part was synthesized by IDT

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
GFP10	1,60	feature/cds, CDS
15aa linker	61,106	sequence_feature, feature/misc
Zif268	119,378	feature/cds, CDS
15aa linker	391,435	CDS, feature/protein
GFP11	436,501	feature/cds, CDS
RBS	502,531	feature/rbs, ribosome_entry_site
GFP9	532,1122	feature/cds, CDS

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1997014/1

Member of these Collections

• iGEM Parts Registry