BBa_K1997022

BBa_K1997022 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1997022

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Chushu Zhu

Date created: 2016-10-12 11:00:00

Date modified: 2016-10-13 07:52:59

P+R->sLuc-N->Zif-N->RBS->Zif-C->sLuc-C->TER

Details

• Types: DnaRegion
• Roles: Composite; engineered_region
• Sequences: BBa_K1997022_sequence (Version 1)

Description

This part is an integrated tool for protein-protein interaction research using split-LUC system as reporter. the "Zif-N->RBS->Zif-C" subpart can be easily replaced using Golden Gate technique with BsaI

Notes

We added BsaI sites at the flank of "Zif-N->RBS->Zif-C" sequence so that this sequence can be easily replaced

Source

This part was synthesized by IDT

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K1789012	1,223	engineered_region, feature/BioBrick
barcode	224,228	feature/barcode, engineered_tag
sLUC-N	229,1476	CDS, feature/cds
10aa linker	1477,1506	CDS, feature/protein
Zif-N	1519,1707	CDS, feature/cds
RBS	1708,1737	feature/rbs, ribosome_entry_site
Zif-C	1738,1815	CDS, feature/cds
10aa linker	1828,1857	feature/cds, CDS
sLUC-C	1858,2316	CDS, feature/cds
barcode	2317,2322	feature/barcode, engineered_tag
BBa_B0015	2323,2451	feature/BioBrick, engineered_region

Other Properties

• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1997022/1

Member of these Collections

• iGEM Parts Registry