Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Shauna Winchester
Date created: 2016-10-11 11:00:00
Date modified: 2016-10-12 05:47:20
psbMZHWK-OPQR
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K1998007_sequence (Version 1) |
Description
This part is composed of two operons in the photosystem II pathway. The psbM, psbZ, psbH, psbW and psbK genes. The psbM protein subunit is positioned at the monomer-monomer interface. The psbZ protein controls the interaction of Photosystem II cores with the light-harvesting antenna. The psbH protein is required for stability and assembly of the photosystem II complex. The psbW protein stabilizes dimeric photosytem II. The psbK protein is also required for stability and assembly of Photosystem II. The second operon is composed of the psbO, psbP, psbQ and psbR genes. The psbO gene that comprises this part ecodes a manganese stabilizing protein. The psbP gene that comprises this part transcribes a protein that optimizes the availability of Ca2+ and Cl- cofactors in the Oxygen Evolving Complex in PSII to maintain the active Manganese cluster. The psbQ protein is an oxygen enhancer protein. The psbR protein is an important link in the Photosystem II core complex for stable assembly of the Oxygen Evolving Complex.Notes
Genes were codon optimised for bacteria using optimiser tool.Restriction sites were identified using Gene Designer, and removed by codon swapping, maintaining AA sequence.
Source
The genes were sourced from Chlamydomonas rienhardtii and synthesised.| Access | Instance | Definition |
| public public | BBa_K1998004 BBa_K1998006 | BBa_K1998004 BBa_K1998006 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K1998004 BBa_K1998006 | 1,1057 1066,3653 | BBa_K1998004 BBa_K1998006 |