Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Royah Vaezi, Kun Xue
Date created: 2009-08-30 11:00:00
Date modified: 2015-05-08 01:11:21
Temperature Sensitive Lamda cI Repressor
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K200011_sequence (Version 1) |
Description
The temperature sensitive lambda cI protein has the cI857 mutation. Therefore, the repressor is denatured at 42??C.It normally represses promoter activity from bacteriophage lambda PL and PR promoters, and has maximal activity at the permissive temperature of 30??C. cI1
However, when the temperature is raised further, typically 42??C, the functionality of such protein is lost and it is no longer able to bind the operator of the promoter. cI2
Notes
NilSource
pGW7 plasmid from ATCC.| Access | Instance | Definition |
| public public public public | BBa_B0034 BBa_K200016 BBa_B0010 BBa_B0012 | BBa_B0034 BBa_K200016 BBa_B0010 BBa_B0012 |