BBa_K200015

BBa_K200015 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K200015

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Royah Vaezi

Date created: 2009-10-13 11:00:00

Date modified: 2015-05-08 01:11:21

Coding region for the DpnII restriction enzyme.

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K200015_sequence (Version 1)

Description

This is a Type II restriction enzyme that recognises the nucleotide sequence 'GATC.' Its activity is blocked by dam methylation.
The working temperature is 37??C.


DpnII is a 4 base cutter, leaving a 4 base overhang.



The cutting site is as shown:



[[Image:II09_DpnII.png]]

Notes

NOTE: Unlike other restriction enzymes that show Star Activity at high pH 8.0, DpnII has Star Activity above approximately pH 6.5. The effect of pH on Star Activity of DpnII is very extreme, showing one-thousand-fold more Star Activity at pH 7.5 than at pH 6.0.

Source

Synthesised from the genomic sequence.

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K200015/1

Member of these Collections

• iGEM Parts Registry