Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Tora H??vermark
Date created: 2016-09-28 11:00:00
Date modified: 2016-09-29 11:23:04
Inducible Green Fluorescent Protein UnaG+Flexible Linker
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K2003012_sequence (Version 1) |
Description
The inducible green fluorescent protein UnaG is 15.6 kDa, i.e. around half the size of GFP. It has maximum absorption at 498 nm and emits light at 527 nm. UnaG fluoresces only when the heme metabolite bilirubin is added (in its unconjugated form), either to a protein solution or to bacterial cells exogenously. The flexible linker is six amino acids long, added to the C terminus, enabling fusion of UnaG to other proteins.Notes
The original UnaG sequence was ordered from IDT with a 6xhistidine tag on the 5' end and a flexible linker on the 3' end. However, the first two codons before the flexible linker were stop codons, meaning that the default UnaG does not translate the flexible linker. The histidine tag and the stop codons were removed from the sequence by PCR, resulting in a UnaG sequence without the histidine tag and with the flexible linker.Source
Synthesized by PCR mutagenesis from UnaG sequence designed and ordered from IDT.| Sequence Annotation | Location | Component / Role(s) |
| Flexible linker UnaG | 425,442 1,424 | CDS feature/cds CDS feature/cds |