Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Rachelle Varga, Nishi Patel
Date created: 2016-10-07 11:00:00
Date modified: 2016-10-08 03:14:00
Bowman Birk Protease Inhibitor (BBI) active nonamer
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K2008000_sequence (Version 1) |
Description
This part contains an active nonamer from the Bowman Birk Protease Inhibitor (BBI) with the addition of lysine, serine, cysteine, and isoleucine (KSCI) tag on the N-terminal end and a phenylalanine (F) tag on the C-terminal end to increase BBI solubility. This component of the composite part is fused to the N-terminal end of GFP part BBA_E0040 for visual detection, and the entire part is under the control of the constitutive E. coli promoter BBa_J23100 and the RBS BBa_B0034.Notes
We used a strong RBS and constitutive promoter to produce high levels of expression of the BBI-KSCI tagged protein fused to GFP for visualization.Source
Members of the lentil family produce the full BBI protein, and the genetic sequence for the peptide was sourced originally from genomic DNA.| Sequence Annotation | Location | Component / Role(s) |
| BBa_J23100 conserved BBa_B0034 GFP protein BBa_E0040 | 1,35 48,51 44,55 62,781 62,781 | feature/BioBrick engineered_region conserved_region feature/conserved feature/BioBrick engineered_region feature/protein CDS feature/BioBrick engineered_region |