Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jun Li
Date created: 2016-10-06 11:00:00
Date modified: 2016-10-15 07:25:11
PcpcG2-B0034-CheZ-GS3-GFP
| Types | DnaRegion |
| Roles | Generator engineered_region |
| Sequences | BBa_K2012019_sequence (Version 1) |
Description
This device is composed with a CcaR-P activated promoter and a GFP fused CheZ protein which is sensitive in bacterial motility,linked behind it.It can generate CheZ stablely and We can detect the expression of cheZ through the flurescence of GFP.
We operate the part in the PJT119b plasmid which comes from Tabor's lab.
Notes
BBa_K2012015+B0034+CheZ-GFP(BBa_K2012008).Here,inside of the CheZ-GFP contain a 45bps 3*(GGGGS) linker.
Source
PcpcG2 promoter is a 238bp green-light activated promoter in Synechocystis PCC 6803(Part:BBa_K592003).The whole sequence is from Synechocystis PCC 6803's genome.We get it from PJT119b plasmid.| Access | Instance | Definition |
| public public public | BBa_K592003 BBa_B0034 BBa_K2012008 | BBa_K592003 BBa_B0034 BBa_K2012008 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K592003 BBa_B0034 BBa_K2012008 | 1,238 247,258 265,1671 | BBa_K592003 BBa_B0034 BBa_K2012008 |