Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Lei Sun
Date created: 2011-09-16 11:00:00
Date modified: 2015-05-08 01:12:48
PcpcG2 promoter, ccaR-regulated
| Types | DnaRegion |
| Roles | Regulatory promoter |
| Sequences | BBa_K592003_sequence (Version 1) |
Description
PcpcG2 is the green-light activated promoter from the genome of Synechocystis PCC6803. This 238 bp sequence is used by Jeffrey Tabor in pJT122 plasmid and contains the entire region upstream of cpcG2 and downstream of ccaR.PcpcG2 is regulated by the light-activation of ccaS/ccaR. The gene downstream to this promoter is transcribed upon activation by ccaS/ccaR, via green light.
Notes
This sequence was PCR-amplified directly from the pJT122 plasmid provided by Chris Voigt, the co-author of Tabor's paper on multichromatic light-sensing (Tabor et al. 2011). Due to its nature, optimizing primers to extract this sequence from the source plasmid was difficult. Recommend touchdown-PCR.Source
This sequence can be amplified from the genome of Synechocystis PCC6803.Reference: Hirose Y, Shimada T, Narikawa R, Katayama M, Ikeuchi M (2008)
Cyanobacteriochrome CcaS is the green light receptor that induces the expression of
phycobilisome linker protein. Proc Natl Acad Sci U S A 105: 9528-9533.
The 238 bp sequence can be directly taken from the pJT122 plasmid designed by Jeffrey Tabor.
Reference: Tabor, J. J., Levskaya, A., and Voigt, C. A. (2011). Multichromatic control of gene
expression in Escherichia coli. J. Mol. Biol. 405, 2, 315???324.
| Sequence Annotation | Location | Component / Role(s) |
| PcpcG2 promoter | 1,238 | promoter feature/promoter |