Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jun Li
Date created: 2016-10-06 11:00:00
Date modified: 2016-10-07 02:50:55
PcpcG2-172-B0034-CheZ-GS3-GFP
| Types | DnaRegion |
| Roles | Generator engineered_region |
| Sequences | BBa_K2012022_sequence (Version 1) |
Description
This device is composed with a CcaR-P activated promoter which removed a constitutive promoter inside it and a GFP fused CheZ protein which is sensitive in bacterial motility,linked behind it. It can generate CheZ stablely and We can detect the expression of cheZ through the flurescence of GFP. We operate the part in the PJT119b plasmid which comes from Tabor's lab.Notes
BBa_K2012015+B0034+CheZ-GFP(BBa_K2012008). Here,inside of the CheZ-GFP contain a 45bps 3*(GGGGS) linker.Source
PcpcG2-172 promoter is a 172bp truncated cpcG2 promoter deleted for the constitutive promoter.It come from PcpcG2 in pJT119b plasmid.| Access | Instance | Definition |
| public public public | BBa_K2012015 BBa_B0034 BBa_K2012008 | BBa_K2012015 BBa_B0034 BBa_K2012008 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K2012015 BBa_B0034 BBa_K2012008 | 1,172 181,192 199,1605 | BBa_K2012015 BBa_B0034 BBa_K2012008 |