BBa_K2012028

BBa_K2012028 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K2012028

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Boyao Zhang

Date created: 2016-10-06 11:00:00

Date modified: 2016-10-07 04:38:15

PleD-His(6)Tag

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K2012028_sequence (Version 1)

Description

PleD-His(6)Tag is the fusion protein of PleD(BBa_K2012002) and His-tag(BBa_J18909). PleD is a response regulator with a diguanylate cyclase (DGC) domain,an enzyme that turns GTP into c-di-GMP. With the fusion of his-tag,PleD protein can be easily detected and purified.

Notes

His(6)tag is added to PleD so that the recombinant protein can be purified through Ni-chelating affinity chromatography.

Source

PleD from Caulobacter crescentus, a response regulator with a diguanylate cyclase (DGC) domain.(Part:BBa_K2012002)

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
PleD	1,1371	feature/cds, CDS
His-tag	1372,1389	feature/cds, CDS

Other Properties

• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K2012028/1

Member of these Collections

• iGEM Parts Registry