BBa_K2012028

BBa_K2012028 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K2012028
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Boyao Zhang
Date created: 2016-10-06 11:00:00
Date modified: 2016-10-07 04:38:15

PleD-His(6)Tag



Types
DnaRegion

Roles
CDS

Coding

Sequences BBa_K2012028_sequence (Version 1)

Description

PleD-His(6)Tag is the fusion protein of PleD(BBa_K2012002) and His-tag(BBa_J18909). PleD is a response regulator with a diguanylate cyclase (DGC) domain,an enzyme that turns GTP into c-di-GMP. With the fusion of his-tag,PleD protein can be easily detected and purified.

Notes

His(6)tag is added to PleD so that the recombinant protein can be purified through Ni-chelating affinity chromatography.

Source

PleD from Caulobacter crescentus, a response regulator with a diguanylate cyclase (DGC) domain.(Part:BBa_K2012002)

Sequence Annotation Location Component / Role(s)
PleD
His-tag
1,1371
1372,1389
feature/cds CDS
feature/cds CDS
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K2012028/1