Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Przemyslaw Nuc
Date created: 2016-10-10 11:00:00
Date modified: 2016-10-14 06:01:45
pBAD-E15'UTR->sfGFP
| Types | DnaRegion |
| Roles | Generator engineered_region |
| Sequences | BBa_K2014000_sequence (Version 1) |
Description
In commercially available expression vectors AraC-pBAD promoter is used. We have found that pBAD without the AraC ORF is as efficient as AraC-pBAD and functional as well. The pBAD promoter can be induced with 0,2% arabinose. We have tested 3 5'UTR variants different from its original 5'UTR, and we have found that E1 5'UTR enhanced the gene expression level upon induction with arabinose.Notes
One of our goals was to find the optimal or the best performing and universal 5'UTR for several E. coli derived inducible promoters in our multipromoter expression system.Source
pBAD promoter is a fragment of Eschericha coli genome; 5'UTR is synthetic, similar to a transcript of T7 bacteriophage,sfGFP is synthetic, based on the publication of Jean-Denis Pedelacq et all (2006).| Sequence Annotation | Location | Component / Role(s) |
| O2 region O1 region I1, I2 binding site -10 signal E1-5'UTR htsfGFP CAP binding site -35 signal 2nd RBS RBS His tag | 1,16 158,179 205,247 269,273 284,322 323,1072 202,212 245,249 298,306 310,315 323,352 | feature/operator operator feature/operator operator feature/binding non_covalent_binding_site sequence_feature feature/misc sequence_feature feature/misc feature/protein CDS non_covalent_binding_site feature/binding feature/misc sequence_feature feature/rbs ribosome_entry_site ribosome_entry_site feature/rbs feature/tag tag |