Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Daria Niewiadomska
Date created: 2016-10-11 11:00:00
Date modified: 2016-10-14 02:17:45
pxylS-M5'UTR->sfGFP
| Types | DnaRegion |
| Roles | Generator engineered_region |
| Sequences | BBa_K2014004_sequence (Version 1) |
Description
Genes involved in catabolism of xylose in E.coli are organized into two transcription units, which drive the transcription in opposite directions, by two promoters - XylA and XylF.During last iGEM edition, we performed many modifications of the initial promoter. XylA1 promoter (BBa_K1741007) was built by changing the original 5???UTR in XylA promoter for 5???UTR derived from constitutive proD promoter.
The next step was to reduce the promoter???s size by removing its XylF part while keeping the modified 5'UTR (from proD) in XylA part- XylS promoter (BBa_K1741009).
And this promoter became our base for XylS-UTR promoter construction, which consist of XylA part with edited 5???UTR. As above in the Ara1-UTR promoter, here again we replaced 5???UTR with synthetic 5???UTR, derived from Mel2 promoter(BBa_K1741004).
Notes
We decided to work on xylose induced promoter cause this sugar is relatively cheap.Source
Xylose induced promoter is a fragment of E.coli K-12 genome. Synthetic 5'UTR is a synthetic unstructured sequence with well positioned RBS.| Sequence Annotation | Location | Component / Role(s) |
| XylR binding site XylR binding site -35 signal -10 signal CRP binding site His - Tag M 5'UTR ht-sfGFP | 20,34 40,55 56,60 79,84 1,19 120,149 93,119 120,869 | feature/binding non_covalent_binding_site non_covalent_binding_site feature/binding feature/misc sequence_feature feature/misc sequence_feature non_covalent_binding_site feature/binding tag feature/tag feature/misc sequence_feature feature/protein CDS |