Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Daria Niewiadomska
Date created: 2016-10-11 11:00:00
Date modified: 2016-10-14 02:32:55
AraC-pBAD->sfGFP_B
| Types | DnaRegion |
| Roles | engineered_region Generator |
| Sequences | BBa_K2014005_sequence (Version 1) |
Description
We were using standard AraC-pBAD promoter to compare the influence of different ORFs structures coding for the same protein sfGFP.Notes
We were looking for the best method to optimize gene expression in E.coli.Source
The AraC-pBAD promoter is a fragment of E.coli K-12 genome. We used one of the sfGFP permutation, with optimized codon sequence.| Sequence Annotation | Location | Component / Role(s) |
| AraC CDS O2 region O1 region -10 signal i1-i2 region 5'UTR ht-sfGFP-B -35 signal CAP binding site His-Tag | 1,879 909,924 1066,1087 1177,1181 1113,1155 1191,1217 1218,1967 1153,1157 1111,1121 1218,1247 | CDS feature/cds operator feature/operator feature/operator operator sequence_feature feature/misc feature/binding non_covalent_binding_site feature/misc sequence_feature CDS feature/protein sequence_feature feature/misc non_covalent_binding_site feature/binding tag feature/tag |