Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Daria Niewiadomska
Date created: 2016-10-11 11:00:00
Date modified: 2016-10-12 02:52:53
AraC-pBAD->sfGFP_8R
| Types | DnaRegion |
| Roles | engineered_region Generator |
| Sequences | BBa_K2014007_sequence (Version 1) |
Description
We were using standard AraC-pBAD promoter to compare the influence of different ORFs structures coding for the same protein sfGFP and also the stability of the mRNA during translation.Notes
We were looking for the best method to optimize gene expression in E.coli and wanted to check out if there is any influence of the 8R tag for the mRNA stability.Source
The AraC-pBAD promoter is a fragment of E.coli K-12 genome. We used one of the sfGFP permutation, with 8R tag to check the translation efficiency.| Sequence Annotation | Location | Component / Role(s) |
| O2 region O1 region -10 signal AraC CDS i1-i2 region 5'UTR ht-sfGFP-8R CAP binding site -35 signal His-Tag 8R | 909,924 1066,1087 1177,1181 1,879 1113,1155 1191,1217 1218,1991 1111,1121 1153,1157 1218,1247 1962,1986 | feature/operator operator operator feature/operator feature/misc sequence_feature feature/cds CDS feature/binding non_covalent_binding_site sequence_feature feature/misc CDS feature/protein non_covalent_binding_site feature/binding sequence_feature feature/misc tag feature/tag feature/misc sequence_feature |