Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Daria Niewiadomska
Date created: 2016-10-11 11:00:00
Date modified: 2016-10-14 10:53:23
pxylF-xylA->sfGFP_B
| Types | DnaRegion |
| Roles | engineered_region Generator |
| Sequences | BBa_K2014011_sequence (Version 1) |
Description
We were using xylF-xylA promoter to compare the influence of different ORFs structures coding for the same protein sfGFP.Notes
We were looking for the best method to optimize gene expression in E.coli.Source
The xylF-xylA promoter is a fragment of E.coli K-12 genome. We used one of the sfGFP permutation, with optimized codon sequence.| Sequence Annotation | Location | Component / Role(s) |
| -10 signal -35 signal IF2 XylR binding site XylR binding site -35 signal -10 signal 5'UTR IF1 CRP binding site 5'UTR ht-sfGFP-B His-Tag | 69,74 91,95 103,118 254,268 274,289 290,294 313,318 1,62 123,138 235,253 324,365 366,1115 366,395 | sequence_feature feature/misc sequence_feature feature/misc sequence_feature feature/misc feature/binding non_covalent_binding_site non_covalent_binding_site feature/binding feature/misc sequence_feature feature/misc sequence_feature feature/misc sequence_feature feature/misc sequence_feature non_covalent_binding_site feature/binding feature/misc sequence_feature CDS feature/protein tag feature/tag |