Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Daria Niewiadomska
Date created: 2016-10-11 11:00:00
Date modified: 2016-10-14 07:26:29
pxylF-xylA->sfGFP_W
| Types | DnaRegion |
| Roles | engineered_region Generator |
| Sequences | BBa_K2014012_sequence (Version 1) |
Description
We were using pxylF-xylA promoter to compare the influence of different ORFs structures coding for the same protein sfGFP.Notes
We were looking for the best method to optimize gene expression in E.coli.Source
The xylF-xylA promoter is a modified version of xylR-xylF-xylA promoter present in E.coli K-12 genome. We used one of the sfGFP permutation, with optimized codon sequence.| Sequence Annotation | Location | Component / Role(s) |
| 5"UTR -10 signal -35 signal IF2 IF1 CRP binding site XylR binding site XylR bidnig site -35 -10 5'UTR His tag ht-sfGFP | 1,62 69,74 91,95 103,118 123,138 235,253 254,268 274,289 290,294 318,318 324,365 366,395 366,1115 | sequence_feature feature/misc sequence_feature feature/misc sequence_feature feature/misc sequence_feature feature/misc feature/misc sequence_feature feature/binding non_covalent_binding_site non_covalent_binding_site feature/binding non_covalent_binding_site feature/binding sequence_feature feature/misc sequence_feature feature/misc sequence_feature feature/misc tag feature/tag feature/protein CDS |