| Types | DnaRegion
|
| Roles | Regulatory
promoter
|
| Sequences | BBa_K202002_sequence (Version 1)
|
Description
Promoter was designed according to the Lutz R and Bujard H.(Nucleic Acids Res. 1997 Mar 15;25(6):1203-10). The operator sequences are from three unrelated natural regulatory elements: the tetracycline (tet), lactose (lac) and λ-phage operons arranged logically within a single transcriptional unit. The regulatory architecture is designed such that each operator's position efficiently interferes with RNA polymerase (RNAp) promoter binding without inhibiting promoter function. This promoter has tetO2 sites which are having transverse mutation at position 5. The hybrid promoter is cloned upstream to the GFP (part Bba_E0240) in plasmid pSBA1. It is ready for assay.
Notes
The regulatory architecture is designed such that each operator's position efficiently interferes with RNA polymerase (RNAp) promoter binding without inhibiting promoter function.
Source
synthesized using tetO2 (mutation at position 5) and lacO1.
