BBa_K2020040

BBa_K2020040 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K2020040

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Andrea Hoeltken, Carolina Bonerath, Vroni Czotscher

Date created: 2016-10-13 11:00:00

Date modified: 2016-12-02 08:35:55

Fluorescent reporter for measurement of incorporation of ncAA

Details

• Types: DnaRegion
• Roles: Reporter; engineered_region
• Sequences: BBa_K2020040_sequence (Version 1)

Description

pRXG is one part of a two plasmid reporter system for measurement of incorporation of ncAA via amber supression. It consists of two flourescent domains connected through a linker sequence containing and amber stop. When IPTG induced and expressed, the flourescence intensity can be measured. A red flourescence is always visible upon induction, and if an amino acid is incorporated as response to the amber stop codon, then a green flourescence intensity is measurable. Fidelty and efficiacy of the incorporation can be determined with comparison of flourescent level.

Notes

This part contains RFP connected with GFP through a linker sequence.

Source

Obtained via the iGEM Team Austin, Texas 2014.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
mRFP1	1,674	feature/protein, CDS
modified Waldo et al linker	678,711	feature/misc, sequence_feature
TAG	712,714	stop_codon, feature/stop
TAA	1435,1437	feature/stop, stop_codon
sfGFP	715,1434	feature/protein, CDS

Other Properties

• igem#experience: None
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K2020040/1

Member of these Collections

• iGEM Parts Registry