BBa_K2020042

BBa_K2020042 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K2020042

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Andrea Hoeltken, Vroni Czotscher, Carolina Bonerath

Date created: 2016-10-01 11:00:00

Date modified: 2016-10-16 04:54:27

Mj-tRNA with amber anticodon for incorporating ncAA in E.coli

Details

• Types: DnaRegion
• Roles: RNA; mature_transcript_region
• Sequences: BBa_K2020042_sequence (Version 1)

Description

For incorporating unnatural amino acids into a protein, a orthogonal tRNA:Synthetase-pair is needed. This tRNA derives from wild type tyrosyl Methanococcus janaschii tRNA:Synthetase pair. It is used together with a tRNA-Synthetase in E.coli. The tRNA has an amber anticodon for incorporating the ncAA in response to an amber codon. It has been proven to work with (enter links for parts) wild type Y-RS, AzF-RS, CNF-RS, Iodo-Y-RS, 5HTP-RS, Nitro-Y-RS by iGEM-Team Texas 2014 (Link).

Notes

Since tRNA and Synthetases have own promotors and since they are frequently used in low copy plasmids, you should assemble tRNA and Syntehtases on a plasmid (e.g. pACYC) which fits your needs.

Team Aachen 2016 ( http://2016.igem.org/Team:Aachen ) used tRNA in pACYC with p15A-ORI, Ipp-Promoter, Gent-Resistance. Together with an ncAA-Synthetase (For example: BBa_K2020050) with own promoter.

Source

wild type tyrosyl Methanococcus janaschii tRNA:Synthetase-pair with anticodon changed to Amber Anticodon CTA

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
tRNA	1,77	feature/misc, sequence_feature

Other Properties

• igem#experience: None
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K2020042/1

Member of these Collections

• iGEM Parts Registry