BBa_K2020050

BBa_K2020050 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K2020050

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Andrea Hoeltken, Carolina Bonerath, Vroni Czotscher

Date created: 2016-10-01 11:00:00

Date modified: 2016-12-19 03:13:18

wild type Mj Y-Synthetase for use in E.coli

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K2020050_sequence (Version 1)

Description

This is the wild type tyrosyl synthetase derived from Methanococcus janaschii to be used as a orthogonal synthetase in E.coli. This part can be used together with the cognate tRNA BBa_K2020042 to incorporate Tyrosin in response to an amber stop codon.

tbc

Notes

Since tRNA and Synthetases have own promotors and since they are frequently used in low copy plasmids, you should assemble tRNA and Syntehtases on a low copy plasmid (e.g. pACYC) which fits your needs.

Team Aachen 2016 used synthetases in pACYC with p15A-ORI and Gent-Resistance. Together with a tRNA (BBa_K2020042) with own Ipp-Promoter and Terminator.

Source

wild type tyrosyl Methanococcus janaschii tRNA-Synthetase. Obtained via iGEM Team 2014 Austin, Texas.

Other Properties

• igem#experience: None
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K2020050/1

Member of these Collections

• iGEM Parts Registry