BBa_K2020058

BBa_K2020058 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K2020058
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Andrea Hoeltken, Carolina Bonerath, Vroni Czotscher
Date created: 2016-10-14 11:00:00
Date modified: 2016-12-02 08:54:46

DMNBS-Synthetase for use in E.coli



    • Details

    Types
    DnaRegion

    Roles
    Coding

    CDS

    Sequences BBa_K2020058_sequence (Version 1)

    Description

    This is a DMNBS-synthetase to be used as a orthogonal synthetase in E.coli. This part can be used together with the cognate tRNA BBa_K2020042 to incorporate DMNBS in response to an amber stop codon.

    Notes

    The synthetase was created via mutation library following computational design from Methanococcus janaschii wild type tyrosyl synthetase.

    Source

    Basis for mutation was Methanococcus janaschii wild type tyrosyl synthetase.

    Sequence Annotation Location Component / Role(s)
    DMNBS-RS
    		1,921
    		sequence_feature feature/misc

    igem#experience
    None
     
    igem#status
    Planning
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K2020058/1
     

    Attachments

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