BBa_K2020058

BBa_K2020058 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K2020058
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Andrea Hoeltken, Carolina Bonerath, Vroni Czotscher
Date created: 2016-10-14 11:00:00
Date modified: 2016-12-02 08:54:46

DMNBS-Synthetase for use in E.coli



Types
DnaRegion

Roles
Coding

CDS

Sequences BBa_K2020058_sequence (Version 1)

Description

This is a DMNBS-synthetase to be used as a orthogonal synthetase in E.coli. This part can be used together with the cognate tRNA BBa_K2020042 to incorporate DMNBS in response to an amber stop codon.

Notes

The synthetase was created via mutation library following computational design from Methanococcus janaschii wild type tyrosyl synthetase.

Source

Basis for mutation was Methanococcus janaschii wild type tyrosyl synthetase.

Sequence Annotation Location Component / Role(s)
DMNBS-RS
1,921
sequence_feature feature/misc
igem#experience
None
 
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K2020058/1