BBa_K2022001

BBa_K2022001 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K2022001

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Gavin Sutton, Shivani Shah

Date created: 2016-10-09 11:00:00

Date modified: 2016-10-12 06:39:42

Attachment Protein g3p, N-terminal Domain, His-tag

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K2022001_sequence (Version 1)

Description

A variant of part BBa_2022000, with a C-terminus His-tag for easier antibody-based recognition. Please see that page for full details:

http://parts.igem.org/wiki/index.php?title=Part:BBa_K2022000

Notes

See: http://parts.igem.org/wiki/index.php?title=Part:BBa_K2022000

The his-tag was created by adding six histidine residues just prior to double stop codons that stop translation. The DNA sequence for these six His residues is catcaccatcaccatcac, which is (roughly) E. coli codon optimised by balancing between the two codons for His

Source

See: http://parts.igem.org/wiki/index.php?title=Part:BBa_K2022000

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
start	1,3	feature/start, start_codon
double stop	235,240	feature/stop, stop_codon
his-tag	217,234	feature/misc, sequence_feature

Other Properties

• igem#experience: None
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K2022001/1

Member of these Collections

• iGEM Parts Registry