BBa_K2022003

BBa_K2022003 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K2022003

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Gavin Sutton

Date created: 2016-10-10 11:00:00

Date modified: 2016-10-10 11:02:46

TolR, Periplasmic Domain, His-tag

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K2022003_sequence (Version 1)

Description

A variant of part BBa_2022002, with a C-terminus His-tag for easier antibody-based recognition. Please see that page for full details: http://parts.igem.org/wiki/index.php?title=Part:BBa_K2022002

Notes

See: http://parts.igem.org/wiki/index.php?title=Part:BBa_K2022002

The his-tag was created by adding six histidine residues just prior to double stop codons that stop translation. The DNA sequence for these six His residues is catcaccatcaccatcac, which is (roughly) E. coli codon optimised by balancing between the two codons for His

Source

See: http://parts.igem.org/wiki/index.php?title=Part:BBa_K2022002

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
start	1,3	start_codon, feature/start
double stop	244,249	stop_codon, feature/stop
his-tag	226,243	feature/misc, sequence_feature

Other Properties

• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K2022003/1

Member of these Collections

• iGEM Parts Registry