Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Gavin Sutton
Date created: 2016-10-12 11:00:00
Date modified: 2016-10-13 05:21:56
T7 Expression Construct for g3p N-terminal Domain
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K2022007_sequence (Version 1) |
Description
This part contains g3p, N-terminal Domain (BBa_K2022000) under the control of a strong T7 promoter and RBS (both from BBa_K525998). Also included is double terminator (BBa_B0017).Notes
We opted to use a T7 promoter to express g3p, as in many expression strains of E. coli (those that are DE3), T7 polymerase is IPTG-inducible. This, ultimately, allows the control of g3p expression, which is critical when the protein has the potential to harm the cell if overexpressed to too great an extentSource
An assembly of BBa_K525998 - BBa_K2022000 - BBa_B0017| Access | Instance | Definition |
| public public public | BBa_K525998 BBa_K2022000 BBa_B0017 | pT7 g3p N1 BBa_B0017 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K525998 BBa_K2022000 BBa_B0017 | 1,32 39,260 269,436 | pT7 g3p N1 BBa_B0017 |