Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jessica Mazalo, Daniel Winter
Date created: 2016-10-10 11:00:00
Date modified: 2016-10-11 05:48:13
RFP, with Periplasm-localisation Tag
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K2022010_sequence (Version 1) |
Description
Red Fluorescent Protein (RFP) is widely-used reporter protein with an absorption and emission maxima at 555nm and 585nm, respectively. This part fuses a sec-signal tag to RFP's n-terminus, which helps export the fusion product to the periplasm and thus bias its subcellular localisation there.Notes
Translation begins at the Met of the signal tag, before continuing into the RFP protein. We ultimately decided to keep the Met that begins RFP, as we couldn't find any evidence to suggest it is cleaved off in the mature, fully-processed form.Source
The sequence for RFP was the open reading frame of BBa_J04450. To its n-terminus we affixed a 20AA sec-signal tag, which guides the fusion protein to the periplasm.| Sequence Annotation | Location | Component / Role(s) |
| Sec signal peptide start RFP double stop | 1,66 1,3 67,747 742,747 | feature/tag tag start_codon feature/start sequence_feature feature/misc stop_codon feature/stop |