BBa_K2027053

BBa_K2027053 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K2027053

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Gordon Sun

Date created: 2016-10-23 11:00:00

Date modified: 2016-10-24 02:36:47

H. Brasiliensis SRPP cDNA (codon Optimized)

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K2027053_sequence (Version 1)

Description

This sequence contains the gene for a small rubber particle protein (SRPP) from the rubber tree Hevea brasiliensis. This coenzyme serves as an activator for rubber transferases/prenyltransferases in H. brasiliensis. This protein is involved in rubber elongation.
Characteristics:
??? 612bp ORF
??? 63-bp 59-UTR
??? 181-bp 39-UTR
??? 18bp poly A tail
??? Localized in the cytoplasm

Notes

This sequence has been codon optimized for transfection into E. coli.
Mutation A486T was induced to remove a restriction site.

A FLAG-Lumio tag can be appended to the sequence to allow for visualization and/or purification.

Source

Sequence was sourced from Oh SK, Kang H, Shin DH, et al. Isolation, characterization, and functional analysis of a novel cDNA clone encoding a small rubber particle protein from Hevea brasiliensis. J Biol Chem. 1999;274(24):17132-8. (http://www.ncbi.nlm.nih.gov/nuccore/AF051317)

Other Properties

• igem#experience: None
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K2027053/1

Member of these Collections

• iGEM Parts Registry